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KMID : 0545120170270050947
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 5 p.947 ~ p.955
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Optimization of Herbicidin A Production in Submerged Culture of Streptomyces scopuliridis M40
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Ha Sang-Hyun
Lee Keon-Jin
Lee Sang-Il
Gwak Hyun-Jung
Lee Jong-Hee
Kim Tae-Woon
Choi Hak-Jong
Jang Ja-Young
Choi Jung-Sub
Kim Chang-Jin
Kim Jin-Cheol
Kim Hyeong-Hwan
Park Hae-Woong
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Abstract
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Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to 180 μm, high volumetric production of herbicidin A was obtained with OUR values >137 mg O2/l·h. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of 100?200 μm length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.
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KEYWORD
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Streptomyces scopuliridis M40, herbicidin A, oxygen uptake rate, hyphal length, scale-up
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